Processing and release of the maize phytocytokine Zip1
This study reveals that the maize phytocytokine Zip1 is activated through a spatially separated, two-stage proteolytic pathway involving intracellular arginine-dependent processing by metacaspases to generate the bioactive Ct-PROZIP1 fragment for export, followed by extracellular turnover by cysteine proteases, thereby establishing a multilayered mechanism for precise spatiotemporal control of plant immunity.